《植物生理学报》 2009, 45(11): 1098-1100

新疆野生种与栽培种郁金香的杂交种子无菌萌发和鳞茎形成

王彩霞¹, 欧阳彤¹, 姜彦成^2,*

1 中国林业科学研究院亚热带林业研究所, 浙江富阳311400; 2 新疆大学生命科学与技术学院, 乌鲁木齐830046

通信作者：姜彦成；E-mail: jiangyc804@163.com；Tel: 0991-8522818

摘　要：

新疆野生种与栽培种郁金香的杂交F1种子于4 ℃低温下培养36~67 d后萌发, 随后于15 ℃下培养3~5个月形成鳞茎。‘ 小黑人’ ×柔毛郁金香、‘ 小黑人’ ×天山郁金香和‘ 小黑人’ ×伊犁郁金香F1 种子的萌发率差异显著, 分别为60.37%~63.89%、-180.28%~81.25% 和 3.75%~4.45%。‘ 小黑人’ ×柔毛郁金香和‘ 小黑人’ ×天山郁金香F1 的鳞茎分别在添加6-BA 2.0 mg•L(单位下同)+KT 1.0+TDZ 0.1 和 6-BA 5.0+KT 0.4 的 1/2 MS 培养基中增殖。‘ 小黑人 ’ ×天山郁金香 F1 种子萌发后有 41.67% 呈 畸形, 畸形的萌芽在添加 6-BA 2.0+KT 0.4 的 1/2 MS 培养基中可诱导出鳞茎并增殖。

关键词：郁金香; 种间杂种; 种子萌发; 鳞茎形成

收稿：2009-07-07   修定：2009-10-27

资助：国家自然科学基金(30560016)。

Aseptic Germination and Bulblet Formation of Interspecific Hybrids between Wild Species and Cultivar of Tulip (Tulipa L.)

WANG Cai-Xia¹, OUYANG Tong¹, JIANG Yan-Cheng^2,*

¹Research Institute of Subtropical Forestry, China Academy of Forestry, Fuyang, Zhejiang 311400, China; ²College of Life Science and Technology, Xinjiang University, Urumqi 830046, China

Corresponding author: JIANG Yan-Cheng; E-mail: jiangyc804@163.com; Tel: 0991-8522818

Abstract:

The F₁ seeds of interspecific hybrids between wild species and cultivar of tulip (Tulipa L.) germinated after 36–67 days cultured in 4 ℃ and formed subsequently bulblets after 3–5 months cultured in 15 ℃. The germination rates of F₁ seeds of ‘Negrita’× Tulipa buhseana, ‘Negrita’×T. tianschanica, and ‘Negrita’×T. iliensis were 60.37%–63.89%, 80.28%–81.25%, 3.75%–4.45% respectively and there were significant differences among them. ‘Negrita’×T. buhseana F₁ bulblets proliferated in 1/2MS medium supplemented with 2.0 mg·L^-1 6-BA, 1.0 mg·L^-1 KT and 0.1 mg·L^-1 TDZ, meanwhile, ‘Negrita’×T. tianschanica F₁ bulblets proliferated in 1/2 MS medium supplemented with 5.0 mg·L^-1 6-BA and 0.4 mg·L^-1 KT. There were 41.67% ‘Negrita’×T.tianschanica showed abnormal morphology, which could induce bulblets in 1/2MS medium supplemented with 2.0 mg·L^-1 6-BA and 0.4 mg·L^-1 KT.

Key words: Tulip (Tulipa L.); interspecific hybrid; seed germination; bulblet formation